Standardization of production and purification of Manduca hemolymph JHBP (Abstract)

The abstract of my research project for applying the Hilldale award (my project hasn't have a result and conclusion). Many students have attempted on this project and didn't work out a desirable yield of rJHBP. If my future work is anything useful, the results will contribute to the scientific community. It's kind of hard to believe I'm doing it.

The hemolymph juvenile hormone binding protein (hJHBP) is a specific Lepidopteran protein that binds and transports juvenile hormone (JH). JH plays a number of crucial roles in the regulation of insect development and reproduction. This project will employ a transformed E. coli cell line to express recombinant JHBP (rJHBP). Immunological analysis, cell preparation, and protein purification will be explored to produce and purify rJHBP of optimal quantity and quality. Binding studies will confirm the functionality of rJHBP. The results will provide a stable supply of JHBP for future studies on the binding and target cell docking mechanisms and contribute to increasing our knowledge of insect physiology and pest control.

History Professor

Just thought of how long I haven't been doodling in class. My history of science professor.

好幸福之火腿蛋炒飯

這禮拜每天睡眠不足，上統計學課一直打瞌睡。我想可能是拿到$3000的獎學金的希望讓我撐著，哈！
在Facebook上看到以前的一個高中同學居然晚餐吃電鍋蒸一整顆花菜加三粒魚丸和白飯加鹽；據她本人說不是人吃的。回家做了高麗菜火腿蛋炒飯，把冰箱裡放了一星期快凍成冰米磚的飯用掉，果然飯(美國米比較乾)炒一炒又復活了。一邊吃著這道簡單的家常菜一邊覺得自己好幸福。
今天在廁所撿到某人遺失的學生證和駕照。在用iPad通信的幫助之下，兩小時內順利將證件交還給主人。我想她一定很著急，幫助同學找回遺失的東西感覺真好。
話說學校報社的漫畫版編輯姓Moriarty，每次他寄信來，寄件者都是Moriarty。雖然前面的名字不一樣，我每次看到還是很想笑。

Student Organizations

I'm excited! There are some student organizations that sound very interesting. Some of them are old; some are new. I'll try my best to see which ones I could continue participating.

Besides continuing going to AHA and oSTEM, there's a new group called electronic music production. The group leader is a DJ and people there are all EDM lovers, who may or may not know how to produce music on a computer. Though the majority are male. I don't know how much time I could actually devote into trying the sound manipulation software, but I go there to make friends (lots of dubstep, house, and trance-lovers), and at least learn about the terminology, so I can understand people's conversation on electronic music production.

There is another group that doesn't have meetings, but the leader will arrange you to pair up with a woman in Kenya. Those women are poor people who are learning sewing as a living and English as the second language. Their English might be good or very elementary. If just writing a letter to a person once every few days or few weeks is helping a poor person with limited resources to learn, that sounds like something I'd love to do.

I visited the International Socialist Organization's meeting today. I was curious, and people there seemed to have some interesting ideas and debates. They were friendly too. I might stop by some time during the semester.

There's a Cheese Club that meets every three weeks. They teach you about cheese varieties and let you taste samples. Sadly it conflicts with my other meetings. Oh and the Cyclist Club. It sounds awesome to ride bike with people around Madison for the sake of exercising and relaxing. I could get to know some other parts of Madison too. Of course, we'll have to wait until the weather gets better.