The hemolymph juvenile hormone binding protein (hJHBP) is a specific Lepidopteran protein that binds and transports juvenile hormone (JH). JH plays a number of crucial roles in the regulation of insect development and reproduction. This project will employ a transformed E. coli cell line to express recombinant JHBP (rJHBP). Immunological analysis, cell preparation, and protein purification will be explored to produce and purify rJHBP of optimal quantity and quality. Binding studies will confirm the functionality of rJHBP. The results will provide a stable supply of JHBP for future studies on the binding and target cell docking mechanisms and contribute to increasing our knowledge of insect physiology and pest control.